The MC-Easy™ Minicircle DNA Production Kit enables simple, reproducible, and efficient production of high-quality Minicircle DNA. The finely tuned growth and induction media is optimized for minicircle production, and the included ATP-dependent DNase reagent selectively removes genomic DNA without affecting the quality of the minicircle DNA, for clean and effective minicircle production prep after prep.
Choose from this kit, which comes with the ZYCY10P3S2T E.coli Minicircle Production Competent Cells (Cat.# MN920A-1/MN925A-1), or the kit that does not include competent cells (Cat.# MN910A-1/MN915A-1). For-profit organizations, please see the Product Note at the end of this section.
Our MC-Easy Kits also come with a special minicircle DNA transfection reagent, MC-Fection™, that works well with most cell lines.
About Minicircle Technology- Episomal expression sustained over weeks
- Foreign DNA-free
- More efficient transfections from small plasmid size
- Unlimited insert size
- Optimized coli minicircle production strain
- Works in vitro and in vivo
When you want sustained transgene expression without introducing any foreign DNA—such as for model animal and gene therapy development—Minicircle Technology is a great gene expression option. Produced as small excised, circular DNA fragments from a parental plasmid, the non-viral, episomal Minicircle expression cassette is free of any bacterial plasmid DNA sequences, and comes with a variety of promoter and reporter combinations. Their small size facilitates more efficient transfection than what’s possible with standard-sized plasmids, and, while Minicircles do not replicate with the host cell, expression lasts for 14 days or longer in dividing cells, and can continue for months in non-dividing cells.
Product Note:Parental minicircle plasmids and the ZYCY10P3S2T Producer Bacterial Strain are available for purchase by not-for-profit researchers only. Commercial users may purchase pre-made, ready-to-transfect minicircle DNA only. SBI also offers custom parental plasmid cloning and minicircle DNA production to both not-for-profit and commercial end users—contact services@systembio.com for additional details. For any other purposes, including the ability to buy the parental MC production system, commercial users should contact SBI at tech@systembio.com for further information.
MC-Easy Minicircle DNA Production Kit Components
MC-Easy Minicircle DNA Production Kit Components
| Minicircle DNA re-purification reagent kit (Endotoxin free water, Minicircle-safe DNase, ATP (25mM), 10X DNase buffer, Precipitation Buffer) |
| 5x Minicircle Growth Medium |
| 5x Induction Medium |
| MC-Fection transfection reagent for minicircle DNA |
| Minicircle DNA spin columns |
| Competent cells, minicircle E. coli producer strain ZYCY10P3S2T (Cat. # MN920A-1 and MN925A-1 only, see Product Note for purchasing restrictions) |
